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北京百奥创新代理cellscript在中国的业务。 cellscript北京代理，cellscript华东代理，cellscript华北代理，cellscript华中代理，cellscript专业代理。
CELLSCRIPT™的使命是提供优质的产品和技术，用于制作和使用RNA进行细胞翻译，用于临床研究和治疗。目前的产品包括用于体外转录的试剂盒，使用帽类似物或加帽酶的5'RNA加帽，以及3'RNA聚腺苷酸化，以及用于制备加帽的聚（A）尾部mRNA用于翻译的一体化试剂盒。 在细胞中。
CELLSCRIPT™近推出了INCOGNITO™RNA转录试剂盒，用于体外合成含有修饰核苷的RNA，例如假尿苷（Ψ）和/或5-甲基胞苷（m5C）代替相应的U或C规范核苷（图1）。 这些试剂盒之所以如此命名，是因为带帽，聚腺苷酸化和核苷修饰的RNA产物（称为“INCOGNITO mRNAs"）被wei装，因此它们在转染到表达的哺乳动物细胞时不会像相应的未修饰mRNA那样诱导先天免疫应答。 各种RNA传感器。
cellscript专业代理,部分产品列表：
mRNA Production
T7 mScript™ Standard mRNA Production System (PDF)
Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page. |
C-MSC11610 | 10 reactions | $320 |
C-MSC100625 | 25 reactions | $702 |
In Vitro Transcription
T7-FlashScribe™ Transcription Kit (PDF)
For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in |
C-ASF3507 | 50 reactions | $286 |
T7-Scribe™ Standard RNA IVT Kit (PDF)
For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours. |
C-AS3107 | 50 reactions | $267 |
SP6-Scribe™ Standard RNA IVT Kit (PDF)
For the production of in vitro transcribed RNA with SP6 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 90 µg RNA from 1 µg DNA in 2 hours. |
C-AS3106 | 50 reactions | $267 |
Post-Transcriptional Capping
ScriptCap™ m7G Capping System (PDF)
For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce |
C-SCCE0625 | 25 reactions | $436 |
ScriptCap™ 2'-O-Methyltransferase Kit (PDF)
For conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. Can be used simultaneously with ScriptCap m7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction. |
C-SCMT0625 | 25 reactions | $219 |
ScriptCap™ Cap 1 Capping System (PDF)
For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. |
C-SCCS1710 | 10 reactions | $211 |
Co-Transcriptional Capping
MessageMAX™ T7 ARCA-Capped Message Transcription Kit (PDF)
For co-transcriptional capping of IVT RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 80% correctly capped and 20% uncapped. |
C-MMA60710 | 10 reactions | $323 |
AmpliCap-Max™ T7 High Yield Message Maker Kit (PDF)
For co-transcriptional capping of IVT RNA with T7 RNA polymerase and standard cap analog. A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped. |
C-ACM04037 | 25 reactions | $368 |
AmpliCap™ SP6 High Yield Message Maker Kit (PDF)
For co-transcriptional capping of IVT RNA with SP6 RNA polymerase and standard cap analog. A reaction produces 35 µg RNA from 1 µg DNA in 2 hours. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped. |
C-AC0706 | 25 reactions | $368 |
Post-Transcriptional Tailing
A-Plus™ Poly(A) Polymerase Tailing Kit (PDF)
For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAs |
C-PAP5104H | 50 reactions | $219 |
Modified NTP In Vitro Transcription
INCOGNITO™ T7 Ψ-RNA Transcription Kit (PDF)
For the production of pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours. |
C-ICTY110510 | 10 reactions | $410 |
INCOGNITO™ SP6 Ψ-RNA Transcription Kit (PDF)
For the production of pseudouridine-containing, in vitro transcribed RNA with SP6 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 60 µg RNA from 1 µg DNA in 2 hours. |
C-ICSY110510 | 10 reactions | $410 |
INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit (PDF)
For the production of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours. |
C-ICTMY110510 | 10 reactions | $691 |
INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit (PDF)
For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing, |
C-ICTAMY110510 | 10 reactions | $842 |
mRNA Decapping
Cap-Clip™ Acid Pyrophosphatase (PDF)
For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer. |
C-CC15011H | 100 units | $550 |
RNase Inhibition
ScriptGuard™ RNase Inhibitor (PDF)
Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction. |
C-SRI6310K | 10,000 units | $278 |
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