anti-human IgG2 mAb HP6200, biotinylated
产品介绍
品牌:mabtech
货号:3852-6-250
规格:250 µg
欧元:415
种属反应:Human
特异性:Human IgG2
这种单克隆抗体可在抗原特异性ELISA等方法中灵敏、特异地检测人IgG2。
人IgG1、IgG3、IgG4、IgA、IgE、IgM与小鼠、大鼠、山羊、兔、牛的IgG无交叉反应(小于0.1%)。恒河猴和食蟹猴IgG的交叉反应性小于1%。
Mouse IL-4 ELISpot PLUS (ALP)
品牌:mabtech
货号:3311-4APW-2
规格:2 plates
欧元:445
种属反应:Mouse
该试剂盒非常适合需要方便和敏感分析的用户。本实验是为小鼠IL-4分泌细胞计数而设计的。该试剂盒包括预先涂有单克隆抗体的ELISpot板、生物素化检测抗体、链霉亲和素碱性磷酸酶和准备使用的BCIP/NBT+底物。
预涂板减少了分析时间,并将变异性降低。
小鼠IL-4elispotplus试剂盒也可用于链霉亲和素HRP和TMB底物。
详细产品列表:Mabtech代理
IgG(免疫球蛋白G)是血清中zui feng fu 的Ig同种型,占所有血清免疫球蛋白的约80%。在人类中,存在四种IgG亚类,其具有zui gao 的IgG1血清浓度,接着是IgG2,IgG3和IgG4。在小鼠中,IgG亚类定义为IgG1,IgG2a/c,IgG2b和IgG3。IgG分子由两条重链和两条轻链组成,用于特异性的结合抗原。在对抗原的典型免疫应答中,在初始IgM应答后能诱导高水平的IgG抗体。
ELISpot特点:
酶联免疫斑点实验(Enzyme-Linked Immunospot Assay,ELISpot)是一种高灵敏度的在单细胞水平检测细胞因子或免疫球蛋白分泌的免疫学检测手段。现在被大量应用于特异性免疫感染的调查、癌症、过敏,自身免疫性疾病和疫苗开发等领域。ELISpot检测具有相当高的灵敏度,即当一百万个阴性细胞中有一个阳性细胞,ELISpot也可检测到该阳性细胞。
ELISpot原理:
将细胞置于包被有特异性抗体的96孔板中培养,在有刺激的条件下,细胞分泌的细胞因子或免疫球蛋白会被包被抗体捕获。移除细胞后,被捕获的细胞因子可进一步使用生物素标记的一抗来标识,其后再与酶标记的亲和素作用,并加入底物使其呈色,有反应作用的细胞会留下直径大小不等的染色斑点。
如何计数分泌小鼠IgG的B细胞?北京百奥创新作为专业的生命科学领域解决方案供应商,为您推荐Mabtech品牌的ELISpot相关试剂盒,产品优质,发表多篇高分文章。
产品名称 | 产品货号 | 规格(不带板子) | 产品说明 |
Mouse IgG ELISpot BASIC (ALP) | 3825-2A | 适用于4个板子 | 详情 |
Mouse IgG ELISpot BASIC (HRP) | 3825-2H | 适用于4个板子 | 详情 |
特点和优势:
Tips:
抗原特异性B细胞有两种可供选择的方式计数:在检测步骤中通过用抗原包被或通过使用生物素化的抗原。对于记忆B细胞,我们建议用R848和重组小鼠IL-2(均包括在内)进行预刺激。
如下图,(A)抗原或(B)特异性抗免疫球蛋白的抗体与板结合。然后,通过(A)生物素化的抗IgG或(B)生物素化的抗原检测抗原特异性抗体。后一种方法产生更明显的斑点,并且还减少了所需抗原的量。(C)是ELISpot测定以确定总的IgG。
厂家测试结果:
货号3825-2A,试剂盒含链霉亲和素-ALP,厂家测试结果:
在不存在或存在R848和重组IL-2的情况下,将小鼠脾细胞预孵72小时。然后洗涤细胞,加入ELISpot孔中并分析总IgG的分泌(100,000个细胞/孔,过夜孵育)。
精彩文章鉴赏:
Antigens reversibly conjugated to a polymeric glyco-adjuvant induce protective humoral and cellular immunity. Nature Materials. 2019.
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FS-0915-2 | Human TNF-α/GM-CSF FluoroSpot kit | 2 plates |
FS-0915-10 | Human TNF-α/GM-CSF FluoroSpot kit | 10 plates |
FS-1215-2 | Human IL-1β/GM-CSF FluoroSpot kit | 2 plates |
FS-1215-10 | Human IL-1β/GM-CSF FluoroSpot kit | 10 plates |
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FSP-0110-10 | Human IFN-γ/Granzyme B FluoroSpot kit, pre-coated | 10 plates |
FS-0110-2 | Human IFN-γ/Granzyme B FluoroSpot kit | 2 plates |
FS-0110-10 | Human IFN-γ/Granzyme B FluoroSpot kit | 10 plates |
FS1-10-550 | Human Granzyme B FluoroSpotBASIC (550) | for 4 plates |
FSP-0102-2 | Human IFN-γ/IL-2 FluoroSpot kit, pre-coated | 2 plates |
FSP-0102-10 | Human IFN-γ/IL-2 FluoroSpot kit, pre-coated | 10 plates |
FSP-0107-2 | Human IFN-γ/IL-10 FluoroSpot kit, pre-coated | 2 plates |
FSP-0107-10 | Human IFN-γ/IL-10 FluoroSpot kit, pre-coated | 10 plates |
FSP-010703-2 | Human IFN-γ/IL-10/IL-17A FluoroSpot kit, pre-coated | 2 plates |
FSP-010703-10 | Human IFN-γ/IL-10/IL-17A FluoroSpot kit, pre-coated | 10 plates |
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FSP-011803-10 | Human IFN-γ/IL-22/IL-17A FluoroSpot kit, pre-coated | 10 plates |
FS-0102-2 | Human IFN-γ/IL-2 FluoroSpot kit | 2 plates |
FS-0102-10 | Human IFN-γ/IL-2 FluoroSpot kit | 10 plates |
FS-0103-2 | Human IFN-γ/IL-17A FluoroSpot kit | 2 plates |
FS-0103-10 | Human IFN-γ/IL-17A FluoroSpot kit | 10 plates |
FS-0104-2 | Human IFN-γ/IL-13 FluoroSpot kit | 2 plates |
FS-0104-10 | Human IFN-γ/IL-13 FluoroSpot kit | 10 plates |
FS-0107-2 | Human IFN-γ/IL-10 FluoroSpot kit | 2 plates |
FS-0107-10 | Human IFN-γ/IL-10 FluoroSpot kit | 10 plates |
FS-0108-2 | Human IFN-γ/IL-5 FluoroSpot kit | 2 plates |
FS-0108-10 | Human IFN-γ/IL-5 FluoroSpot kit | 10 plates |
FS-050617-2 | Human IgG/IgA/IgM FluoroSpot kit | 2 plates |
FS-050617-10 | Human IgG/IgA/IgM FluoroSpot kit | 10 plates |
FS-05R06G-2 | Human IgG/IgA FluoroSpot kit | 2 plates |
FS-05R06G-10 | Human IgG/IgA FluoroSpot kit | 10 plates |
FS1-01-550 | Human IFN-γ FluoroSpotBASIC (550) | for 4 plates |
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FSP-010209-10 | Human IFN-γ/IL-2/TNF-α FluoroSpot kit, pre-coated | 10 plates |
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FSP-010308-10 | Human IFN-γ/IL-17A/IL-5 FluoroSpot kit, pre-coated | 10 plates |
FSP-0104-2 | Human IFN-γ/IL-13 FluoroSpot kit, pre-coated | 2 plates |
FSP-0104-10 | Human IFN-γ/IL-13 FluoroSpot kit, pre-coated | 10 plates |
FSP-010708-2 | Human IFN-γ/IL-10/IL-5 FluoroSpot kit, pre-coated | 2 plates |
FSP-010708-10 | Human IFN-γ/IL-10/IL-5 FluoroSpot kit, pre-coated | 10 plates |
FSP-0108-2 | Human IFN-γ/IL-5 FluoroSpot kit, pre-coated | 2 plates |
FSP-0108-10 | Human IFN-γ/IL-5 FluoroSpot kit, pre-coated | 10 plates |
FSP-0109-2 | Human IFN-γ/TNF-α FluoroSpot kit, pre-coated | 2 plates |
FSP-0109-10 | Human IFN-γ/TNF-α FluoroSpot kit, pre-coated | 10 plates |
FS-05R17G-2 | Human IgG/IgM FluoroSpot kit | 2 plates |
FS-05R17G-10 | Human IgG/IgM FluoroSpot kit | 10 plates |
FS-1209-2 | Human IL-1β/TNF-α FluoroSpot kit | 2 plates |
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