product name:Human Peripheral Blood Mononuclear Cells, Single donor, Ultra-pure(人外周血单核细胞,单个供体,超纯);
cat no:ABC-TC3716;
Size/Quantity:1 vial;
Biosafety Level:1;
Shipping Info:Dry Ice;
Storage:Liquid Nitrogen;
Description:AcceGen Mononuclear Cells (MNC) represent the enriched lymphocyte and monocyte fraction of whole blood. They are isolated from umbilical cord blood and adult peripheral blood of healthy donors at AcceGen’s cell culture facility. Mononuclear Cells from cord blood contain a relatively high percentage of primitive progenitor cells,whereas Mononuclear Cells from adult peripheral blood (hMNC-PB) contain large numbers of mature immune cells.
Note: When culturing pooled Peripheral Blood Mononuclear Cells, a bidirectional allogeneic mixed lymphocyte reaction (MLR) is likely to occur during the first 48 hours. This will result in cell activation, blast transformation and T-cell proliferation. Thus, these cells are preferably used for several types of infectious virus tests, including neutralization assays. For HIV-related research, prior CD8+ T-cell depletion is recommended.
Freshly collected whole blood is carefully separated by optimized low-density gradient centrifugation, effectively removing the granulocytes. In order to avoid general cell damage, red blood cells are gently depleted by proprietary techniques instead of using classic osmotic lysis protocols. The obtained ultra-pure AcceGen Mononuclear Cells do not clump after thawing, and they exhibit superior viability and unchanged biological function.
Immediately after isolation, the freshly prepared Mononuclear Cells are cryopreserved using AcceGen’s proprietary,serum-free freezing medium, Cryo-SFM.Each cryo vial contains more than 25 million viable cells after thawing.;
Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;
Application:For research use only.
北京百奥创新代理Accegen在中国的业务。
北京百奥创新科技有限公司:
一、辐射五大服务方向:
国内试剂耗材经销代理;
国外试剂订购(直订或境外代理);
基因组测序(二代、二代+三代);
基因分型(SNP检测):低/中/高通量项目;
生物样本运输;
二、多家授权代理品牌,涉及30多种,经营代理500多个品牌:
Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific......
所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,百奥创新自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。
专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作