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首页 > 产品中心 > > Merck Millipore > Iscove′s Modified Dulbecco′s Medium

Iscove′s Modified Dulbecco′s Medium

简要描述:Iscove′s Modified Dulbecco′s Medium
liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

  • 产品型号:型号齐全
  • 厂商性质:代理商
  • 更新时间:2022-10-18
  • 访  问  量:47
详情介绍

Iscove′s Modified Dulbecco′s Medium

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | hybridoma: suitable;cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:phenol red: yes;glucose: yes;L-glutamine: no.

Application:Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.


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