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Rapid Induction of NSCs:
PSC Neural Induction Medium simplifies the NSC generation workflow by eliminating the need to form EBs or rosettes. By removing this step, PSC Neural Induction Medium enables a more reproducible process with high efficiency neural induction in only 7 days.
Scalable Production of NSCs:
PSC Neural Induction Medium is a robust medium that has been shown to generate over 20 million NSCs from 1 million PSCs. The medium has been tested with multiple hPSC lines including hiPSCs derived from use of Episomal iPSC Reprogramming Vectors and the CytoTune™-iPS Sendai Reprogramming Kit.
Generation of High Quality NSCs:
Neural stem cells generated using PSC Neural Induction Medium have >80% expression of Sox1, Sox2, and Nestin (NSC markers), and <1% expression of Oct4 (pluripotency marker). In addition, expanded NSCs maintained phenotype, exhibited normal karyotype, and could be differentiated into neurons, astrocytes, and oligodendrocytes.