mRNA合成试剂盒(加帽加尾)

Product Name:mRNA合成试剂盒(加帽加尾)

Introduction:

The T7 mScript™ Standard mRNA Production System provides all enzymes and enzyme-related  reagents for making 5'-capped, 3'-polyadenylated mRNA. This mScript Kit includes modules for (i) in vitro transcription of linear double-stranded DNA templates using the mScript T7 Enzyme Solution, the  canonical nucleotides ATP, CTP, GTP and UTP, (ii) enzymatic capping of the RNA using ScriptCap™  Capping Enzyme System (for making mRNA with a Cap 0 cap structure), (iii) ScriptCap™  2'-O-Methyltransferase (for optionally making mRNA with a Cap 1 cap structure) and (iv) A-Plus™ Poly(A)  Polymerase for adding a 3'-poly(A) tail.  Post-transfection, capped and tailed mRNA has increased stability and translation efficiency in most  eukaryotic cell lines. The mScript System improves upon existing capping methods by ensuring virtually  100% transcript capping, all caps in the proper orientation and the ability to produce large amounts of  capped RNA at a reasonable cost. This mRNA is suitable for use in transfection and microinjection  experiments as well as in vitro translation systems.