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cellscript 体外转录试剂盒

简要描述:The T7 mScript™ Standard mRNA Production System(cellscript 体外转录试剂盒)provides all enzymes and enzyme-related reagents for making 5'-capped, 3'-polyadenylated mRNA.

  • 产品型号:型号齐全
  • 厂商性质:代理商
  • 更新时间:2022-11-18
  • 访  问  量:105
详情介绍

Product Name:T7 mScript™ Standard mRNA Production System(cellscript 体外转录试剂盒)

Introduction:

The T7 mScript™ Standard mRNA Production System provides all enzymes and enzyme-related  reagents for making 5'-capped, 3'-polyadenylated mRNA. This mScript Kit includes modules for (i) in vitro transcription of linear double-stranded DNA templates using the mScript T7 Enzyme Solution, the  canonical nucleotides ATP, CTP, GTP and UTP, (ii) enzymatic capping of the RNA using ScriptCap™  Capping Enzyme System (for making mRNA with a Cap 0 cap structure), (iii) ScriptCap™  2'-O-Methyltransferase (for optionally making mRNA with a Cap 1 cap structure) and (iv) A-Plus™ Poly(A)  Polymerase for adding a 3'-poly(A) tail.  Post-transfection, capped and tailed mRNA has increased stability and translation efficiency in most  eukaryotic cell lines. The mScript System improves upon existing capping methods by ensuring virtually  100% transcript capping, all caps in the proper orientation and the ability to produce large amounts of  capped RNA at a reasonable cost. This mRNA is suitable for use in transfection and microinjection  experiments as well as in vitro translation systems.


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