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Related ArticlesProduct Name: Poly(A)加尾试剂盒
Introduction:
The A-Plus™ Poly(A) Polymerase Tailing Kit uses ATP as a substrate for template-independent addition of adenosine monophosphates to the 3'-hydroxyl termini of RNA. The standard protocol produces a poly(A)-tail length of ~150 b on 40-60 μg of RNA. Polyadenylation increases the stability of RNA in eukaryotic cells and enhances its ability to be translated after transfection or microinjection.1-3 A Poly(A) tail is useful to provide a priming site for first-strand cDNA synthesis in certain applications, and can be used to end-label4 or quantify5 mRNA.