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首页 > 产品中心 > 实验试剂 > 美国R&D Systems试剂盒 > Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT)

Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT)

简要描述:产品名称:Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT)
产品货号:M6000B
产品规格:1KT
现货特价促销,数量1,售完为止

  • 产品型号:型号齐全
  • 厂商性质:代理商
  • 更新时间:2017-10-27
  • 访  问  量:713
详情介绍

 现货特价促销Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT),数量1,售完为止

Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT)产品简介:

  • Assay Length

    4.5 hours

  • Sample Type & Volume Required Per Well

    Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)

  • Sensitivity

    1.8 pg/mL

  • Assay Range

    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)

  • Specificity

    Natural and recombinant mouse IL-6

  • Cross-reactivity

    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

  • Interference

    No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accuray quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.

Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT)介绍:

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, Heparin Plasma

 Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020282227
Mean30872322985238
Standard Deviation23.48.11.87.518.1
CV%6.73.93.56.18.97.6

 

Recovery

The recovery of mouse IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.

 

Sample TypeAverage % RecoveryRange %
Cell Culture Supernates (n=5)11086-120
Heparin Plasma (n=6)10181-115
Serum (n=5)9984-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Mouse IL-6 Quantikine ELISA Kit

Preparation and Storage

  • Storage

    Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

    • Long Name

      Interleukin 6

    • Entrez Gene IDs

      3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit);

    • Alternate Names

      B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A;

    Related Research Areas

    • Adipocytokines
    • Cancer Biomarkers
    • Conventional/Classical Dendritic Cells
    • Cytokines and Receptors in Allergy and Asthma
    • Cytokines and Receptors in Angiogenesis
    • Cytokines and Receptors in Tfh Cell Differentiation and Function
    • Diabetes
    • gamma delta T Cells
    • Glucose Homeostasis
    • Helper T (Th) Cells
    • HIF Transcription Factors
    • IL-6 Family
    • Infectious Diseases
    • Inflammatory Mediators
    • Macrophage Activation Markers
    • Molecules Secreted by VSMC
    • Multiple Myeloma
    • Multiple Sclerosis and Myelin Related Molecules
    • Myeloid-derived Suppressor Cells (MDSC)
    • Neuroinflammation
    • Obesity
    • Plasmacytoid Dendritic Cells
    • T Cell Cytokine Signaling
    • Th17 Cells
    • Th22 Cells
    • Th9 Cells

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